In our lab, we utilise cell and molecular approaches to define the mechanisms of vesicle trafficking along the endolysosomal and autophagic pathways. Our research approach encompasses investigating mechanisms of cargo identification and delivery, impacts on signal regulation, and the collective effect on cell phenotype. We utilise siRNA-mediated knockdown and CRISPR-Cas9 gene knockout approaches coupled to reconstitution experiments to determine functional mechanisms. We use biochemical approaches to determine signaling perturbations and investigate protein-protein interactions using BioID or coIP approaches. We also use advanced imaging and analysis tools to explore spatial and temporal subcellular protein localisation as well as to investigate organelle organisation. These approaches provide vital details of the cellular and molecular requirements for membrane and cytoplasmic cargo trafficking and degradation, and their relationship to cell function. Collectively, these investigations provide deeper insight into aspects of cell physiology focused around cell homeostasis, and give molecular details into the pathogenesis of disease, including cancer and neurodegeneration, which manifest as a result of dysfunction in many of these pathways.